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mouse osteogenesis culture medium kit  (Cosmo Bio USA)

 
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    Cosmo Bio USA mouse osteogenesis culture medium kit
    Mouse Osteogenesis Culture Medium Kit, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+osteogenesis+culture+medium+kit/mouse+osteogenesis+culture+kit/10__2485_slash_jhtb__32__213-68-10-15
    Average 90 stars, based on 1 article reviews
    mouse osteogenesis culture medium kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Morphological Study for the Osteocytes in Podoplanin-Conditional Knockout Mice
    Article Snippet: .. Total RNA extraction from the primary cell culture in a mouse osteogenesis culture medium kit (Cosmo Bio) was performed with a QIAshredder column and RNeasy kit (Qiagen, Inc., Tokyo, Japan) and the RT was performed on 30 ng of total RNA. .. The PCR was performed for the gemonic DNA and RT products by 30 cycles of amplification using the Ex Taq hot start version (Takara Bio Inc., Shiga, Japan).

    Article Title: Cancellation of the Calcification in Cultured Osteoblasts by CLEC-2
    Article Snippet: .. Total RNA extraction from the 80% confluent monolayer of osteoblasts (Cosmo Bio) cultured in α-MEM, in a mouse osteogenesis culture medium kit (Cosmo Bio) and in the medium containing 0.1 and 1 μg/ml CLEC-2 (R&D Systems) in collagen-coated 24-well culture plates was performed with a QIAshredder column and RNeasy kit (Qiagen, Inc., Tokyo, Japan). .. Contaminating genomic DNA was removed using DNAfree (Ambion, Huntingdon, UK), and the RT was performed on 30 ng of total RNA, followed by 30 cycles of PCR for amplification using the Ex Taq hot start version (Takara Bio Inc., Otsu, Japan) with 50 pM of primer sets for mouse mRNA of β-actin, podoplanin, osteopontin, osteocalcin, and sclerostin, where the specificities had been confirmed by the manufacturer (Table 1) (Sigma-Aldrich Corp., Tokyo, Japan).

    Cell Culture:

    Article Title: Morphological Study for the Osteocytes in Podoplanin-Conditional Knockout Mice
    Article Snippet: .. Total RNA extraction from the primary cell culture in a mouse osteogenesis culture medium kit (Cosmo Bio) was performed with a QIAshredder column and RNeasy kit (Qiagen, Inc., Tokyo, Japan) and the RT was performed on 30 ng of total RNA. .. The PCR was performed for the gemonic DNA and RT products by 30 cycles of amplification using the Ex Taq hot start version (Takara Bio Inc., Shiga, Japan).

    Article Title: Cancellation of the Calcification in Cultured Osteoblasts by CLEC-2
    Article Snippet: .. Total RNA extraction from the 80% confluent monolayer of osteoblasts (Cosmo Bio) cultured in α-MEM, in a mouse osteogenesis culture medium kit (Cosmo Bio) and in the medium containing 0.1 and 1 μg/ml CLEC-2 (R&D Systems) in collagen-coated 24-well culture plates was performed with a QIAshredder column and RNeasy kit (Qiagen, Inc., Tokyo, Japan). .. Contaminating genomic DNA was removed using DNAfree (Ambion, Huntingdon, UK), and the RT was performed on 30 ng of total RNA, followed by 30 cycles of PCR for amplification using the Ex Taq hot start version (Takara Bio Inc., Otsu, Japan) with 50 pM of primer sets for mouse mRNA of β-actin, podoplanin, osteopontin, osteocalcin, and sclerostin, where the specificities had been confirmed by the manufacturer (Table 1) (Sigma-Aldrich Corp., Tokyo, Japan).

    Recombinant:

    Article Title: Cancellation of the Calcification in Cultured Osteoblasts by CLEC-2
    Article Snippet: Llc., St. Louis, MO) with 10% fetal bovine serum (Biowest, Nuaillé, France). .. For the test of the production of bone cell proteins and calcification, the cells were seeded on collagen-coated 24-well culture plates (Asahi Glass Co., Ltd., Shizuoka, Japan) in calcification medium of a mouse osteogenesis culture medium kit (Cosmo Bio) containing 100 nM dexamethasone, 50 μg/ml, ascorbic acid, and 10mM β-glycerophosphate and further in the medium containing 0.1 and 1 μg/ml the recombinant intact mouse CLEC2 (Catalog#9117-CL-050, R&D Systems Inc., Minneapolis, MN). .. The CLEC-2 binding ability to podoplanin was described in the manufacturer data sheet for the functional ELISA: the CLEC-2 binds with a typical ED50 of 0.1-0.5 μg/ml on recombinant mouse podoplanin (Fc chimera, #3244- PL, R&D Systems) coated at 1.0 μg/ml (100 μl/well) in a 96-well microtitration plate.



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    mouse osteogenesis culture medium kit  (Cosmo Bio USA)
    90
    Cosmo Bio USA mouse osteogenesis culture medium kit
    Mouse Osteogenesis Culture Medium Kit, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+osteogenesis+culture+medium+kit/mouse+osteogenesis+culture+kit/10__2485_slash_jhtb__32__213-68-10-15
    Average 90 stars, based on 1 article reviews
    mouse osteogenesis culture medium kit - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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